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Biochemistry

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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
 

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Article doi: 10.3791/59215
March 5th, 2019

Summary March 5th, 2019

We present experimental approaches for studying RNA-interactors of double-stranded RNA binding protein kinase RNA-activated (PKR) during the mammalian cell cycle using HeLa cells. This method utilizes formaldehyde to crosslink RNA-PKR complexes and immunoprecipitation to enrich PKR-bound RNAs. These RNAs can be further analyzed through high-throughput sequencing or qRT-PCR.

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