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Biochemistry

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JoVE Journal Biochemistry
Estudando o RNA interactianos de proteína quinase RNA-ativado durante o ciclo celular dos mamíferos
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Estudando o RNA interactianos de proteína quinase RNA-ativado durante o ciclo celular dos mamíferos

Article DOI: 10.3791/59215-v • 10:05 min • March 5th, 2019
March 5th, 2019 •
1, 1, 1
1Department of Chemical and Biomolecular Engineering and KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

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Nós apresentamos abordagens experimentais para estudar RNA-interactianos de double-stranded RNA ligação proteína quinase RNA-ativado (PKR) durante o ciclo de pilha mamífero usando células HeLa. Este método utiliza o formaldeído para complexos de crosslink RNA-PKR e imunoprecipitação para enriquecer RNAs ligados a PKR. Estes RNAs podem ser analisados ainda mais através de sequenciamento de alto rendimento ou qRT-PCR.

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Bioquímica edição 145 Double-stranded do RNA do RNA mitocondrial RNA ligação proteína interação RNA-RBP proteína quinase RNA-ativado reticulação de formaldeído ciclo celular vertente específica qRT-PCR
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