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Visualization of the Superior Ocular Sulcus during Danio rerio Embryogenesis
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1Department of Biological Sciences,University of Alberta, 2Women & Children’s Health Research Institute,University of Alberta, 3Division of Anatomy, Department of Surgery,University of Alberta, 4Department of Cell Biology,University of Alberta, 5Department of Medical Genetics,University of Alberta, 6Neuroscience and Mental Health Research Institute,University of Alberta
Chapters
- 00:04Title
- 00:28Superior Ocular Sulcus (SOS) Visualization
- 03:15Whole-Mount Immunofluorescent Laminin Staining
- 06:04Results: Representative Zebrafish SOS Imaging
- 07:31Conclusion
Here, we present a standardized series of protocols to observe the superior ocular sulcus, a recently-identified, evolutionarily-conserved structure in the vertebrate eye. Using zebrafish larvae, we demonstrate techniques necessary to identify factors that contribute to the formation and closure of the superior ocular sulcus.
