Journal
/
Cancer Research
/
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

DOI:
10.3791/59271-v

•

08:07 min

•

April 12, 2019

•

, ,
1MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences,Sun Yat-sen University

Chapters

  • 00:04Title
  • 00:59Bone Preparation and Demineralization
  • 02:29Bone Decellularization
  • 04:08Cell Seeding and Culture
  • 06:26Results: Characterization of Decellularized BEM and OS Cells
  • 07:44Conclusion

Summary

Automatic Translation

Automatic Translation

The bone extracellular matrix (BEM) model for osteosarcoma (OS) is well established and shown here. It can be used as a suitable scaffold for mimicking primary tumor growth in vitro and providing an ideal model for studying the histologic and cytogenic heterogeneity of OS.

Tags

Keywords: 3D Bone Extracellular MatrixOsteosarcomaDecellularizationBone-derived MatrixBone Tumor ResearchHeterogenous MorphologyDrug SensitivityEwing SarcomaMetastatic Bone TumorsBALB/c MiceBone DecalcificationLipid Extraction

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59271?article_type=v

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol
check_url/59271?article_type=v

Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System
check_url/59271?article_type=v

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
check_url/59271?article_type=v

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening
check_url/59271?article_type=v

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
check_url/59271?article_type=v

Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels
check_url/59271?article_type=v

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
check_url/59271?article_type=v

Establishment and Analysis of Three-Dimensional (3D) Organoids Derived from Patient Prostate Cancer Bone Metastasis Specimens and their Xenografts
check_url/59271?article_type=v

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells
check_url/59271?article_type=v

Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

Read Article