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Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans
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Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

DOI:
10.3791/59277-v

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08:10 min

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February 23, 2019

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,
1Department of Regenerative and Cancer Cell Biology,Albany Medical College

Chapters

  • 00:04Title
  • 00:32Growth and Synchronization of Nematode Population
  • 02:40Hydration of Probes, Drug Loading and Cartridge Equilibration
  • 04:40Preparation of Worm Plate, Assay Run, and Post-experiment Data Analysis
  • 06:45Results: OCR in Wild Type and Various sel-12 Mutants
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

Mitochondrial respiration is critical for organismal survival; therefore, oxygen consumption rate is an excellent indicator of mitochondrial health. In this protocol, we describe the use of a commercially available respirometer to measure basal and maximal oxygen consumption rates in live, intact, and freely-motile Caenorhabditis elegans.

Tags

Keywords: Oxygen Consumption RateCaenorhabditis ElegansMitochondrial FunctionOxygen Consumption AssayC. ElegansL4 LarvaeOP50NGM PlatesFUdRRespirometrySynchronization

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