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Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
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Bioengineering
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JoVE Journal Bioengineering
Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

DOI:
10.3791/59285-v

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04:59 min

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April 12, 2019

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,
1Department of Biology,Harper College

Chapters

  • 00:04Title
  • 00:30Medium Preparation
  • 01:28S. aureus Biofilm Formation in 96 Well Tissue Culture Plate
  • 02:48Measurement of Alkaline Phosphatase (ALP) Activity in S. aureus Biofilm
  • 03:45Results: Alkaline Phosphatase Activity
  • 04:10Conclusion

Summary

Automatic Translation

Automatic Translation

In this manuscript, we established a high throughput method to quantify alkaline phosphatase activity in S. aureus biofilm culture from 96-well tissue culture plate

Tags

Alkaline Phosphatase ActivityS. AureusBiofilmColorimetric AnalysisHigh-throughputTSBTSAGlucose96-well PlatePNPPSodium Hydroxide

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