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JoVE Journal
Medicine

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A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents
 

A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

Article doi: 10.3791/59295
April 19th, 2019

Summary April 19th, 2019

Here we present a method to validate tail vein injections in rats by utilizing near-infrared fluorescence imaging data from dyes incorporated into agents or biological probes. The tail is imaged before and after the injection, the fluorescent signal is quantified, and an assessment of the injection quality is made.

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