Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
 

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Article DOI: 10.3791/59297-v 09:54 min April 18th, 2019
April 18th, 2019

Chapters

Summary

We propose a protocol that shows how to differentiate induced pluripotent stem cell-derived keratinocytes and fibroblasts and generate a 3D skin organoid, using these keratinocytes and fibroblasts. This protocol contains an additional step of generating a humanized mice model. The technique presented here will improve dermatologic research.

Tags

3D Skin Organoid Cord Blood-derived Induced Pluripotent Stem Cells CBMC CMBC-iPSCs Keratinocytes Fibroblasts Vitronectin-coated Plates Culturing EDTA E8 Medium Centrifugation Embryonic Body Formation Medium ROCK Inhibitor
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter