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Measuring Enzymatic Stability by Isothermal Titration Calorimetry
 

Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Article doi: 10.3791/59302
March 26th, 2019

Summary March 26th, 2019

The thermal stability of enzyme activity is readily measured by isothermal titration calorimetry (ITC). Most protein stability assays currently used measure protein unfolding, but do not provide information about enzymatic activity. ITC enables direct determination of the effect of enzyme modifications on the stability of enzyme activity.

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