Journal
/
Biochemistry
/
A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput

DOI:
10.3791/59333-v

•

00:12 min

•

September 22, 2019

•

, , ,
1National Institute of Neurological Disorders and Stroke (NINDS), Neurotherapeutic Development Unit (NTDU),National Institutes of Health (NIH)

Chapters

  • 00:04Title
  • 00:58Dissociate and Plate Neural Stem Cells
  • 03:12Treating Cells with Compounds
  • 06:10Imaging Cells on a Plate Reader
  • 08:27Terminal MTT (3-(4,5-dimethylthizol-2-yl)-2,5-diphenyltetrazolium bromide) Cytotoxicity Assay
  • 10:04Results: tdTomato Assay and MTT Assay
  • 11:40Conclusion

Summary

Automatic Translation

Automatic Translation

It is often necessary to assess the potential cytotoxicity of a set of compounds on cultured cells. Here, we describe a strategy to reliably screen for toxic compounds in a 96-well format.

Tags

Keywords: Cell GrowthCell SurvivalCell CultureCompound ScreeningCytotoxicityCell ProliferationCell ViabilityHigh-throughputLow-to-moderate ThroughputMulti-assay StrategyCell DissociationCell Counting96-well PlateExtracellular Matrix CoatingCell Culture Incubation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59333?article_type=v

Measuring Protein Binding to F-actin by Co-sedimentation
check_url/59333?article_type=v

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
check_url/59333?article_type=v

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
check_url/59333?article_type=v

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
check_url/59333?article_type=v

In Vivo Calcium Imaging in C. elegans Body Wall Muscles
check_url/59333?article_type=v

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
check_url/59333?article_type=v

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time
check_url/59333?article_type=v

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
check_url/59333?article_type=v

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
check_url/59333?article_type=v

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography (Video) | JoVE

Read Article