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Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy

JoVE Journal
Biology

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JoVE Journal Biology

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy

DOI:

10.3791/59335-v

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07:40 min

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August 09, 2019

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Rafael M. Rezende*¹, Mateus E. Lopes*², Gustavo B Menezes, Howard L. Weiner

¹Ann Romney Center for Neurologic Diseases,Brigham and Women’s Hospital, Harvard Medical School, ²Center for Gastrointestinal Biology,Federal University of Minas Gerais

Chapters

00:04Title
00:56Antibody Cocktail Injection
01:45Inguinal Draining Lymph Node (LN) Harvest
02:49Popliteal Draining LN Harvest
03:41LN Preparation and Ex-Vivo Confocal Microscopy Imaging
05:11Results: Representative Whole LN Confocal Microscopy Imaging
07:12Conclusion

Summary

Automatic Translation

This protocol describes a technique to image different cell populations in draining lymph nodes without alterations in the organ structure.

Tags

Keywords: Lymph Node Ex-vivo Confocal Microscopy Fluorescent Antibodies Mouse Handling Inguinal Lymph Node Popliteal Lymph Node Microsurgery Skin Incision Cell Viability

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