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Biochemistry

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JoVE Journal Biochemistry
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
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Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Article DOI: 10.3791/59336-v • 09:50 min • August 14th, 2019
August 14th, 2019 •
1, 1, 1, 1, 1, 1, 5, 1, 1,2,3,4
1College of Bioscience and Biotechnology, Yangzhou University, 2Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, 3Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, 4Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 5The Testing Center, Yangzhou University

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Summary
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The derivation of a flavonol is crucial for its application in healthcare and the food industry. Here, we provide a detailed protocol for the biosynthesis of a flavonol from a flavanone and discuss the crucial steps and its advantages over other approaches.

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Biosynthesis Flavonol Flavanone Bienzymatic Cascade One-pot Reaction Industrialization Potential Secondary Metabolites Yield Improvement Recombinant Enzymes Buffer Preparation TRIS-base Sodium Ascorbate Alpha Ketoglutaric Acid Glycerol Hydrochloric Acid PH Adjustment Ferrous Sulfate Heptahydrate Flavonoid Synthesis
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