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En Orthotopic livmor Cancer modell med retroperitoneal lymfadenopati laget fra in vivo spredte og kultivert VX2 Cells
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1Institute of Medical Science,University of Toronto, 2Department of Obstetrics and Gynecology,University of Toronto, 3Guided Therapeutics Laboratory, TECHNA Institute,University Health Network, 4Department of Pathology, Princess Margaret Cancer Center,University Health Network, 5Department of Medical Biophysics,University of Toronto, 6Princess Margaret Cancer Center,University Health Network, 7Techna Institute,University Health Network, 8Department of Surgery,University of Toronto, 9Department of Otolaryngology, Head and Neck Surgery,University of Toronto, 10Division of Gynecologic Oncology, University of Toronto /Princess Margaret Cancer Center,University Health Network, 11Princess Margaret Cancer Center,University Health Network
Chapters
- 00:04Title
- 00:59In Vitro-Propagated VX2 Cell Suspension Preparation
- 02:35In Vivo-Propagated VX2 Cell Suspension Preparation
- 03:27Surgical Field Preparation
- 04:17Laparotomy and Uterine Horn Identification
- 05:31Myometrial VX2 Cell Inoculation
- 06:21Surgical Incision Closure and Post-Operative Care
- 08:03Results: VX2 Tumor Establishment and Characterization
- 09:09Conclusion
Denne protokollen presenterer en standardisert metode for å vokse VX2 celler i kultur og å skape en orthotopic VX2 modell av livmorkreft med retroperitoneal lymfe node metastaser i kaniner. Orthotopic livmorkreft modeller er viktig for pre-klinisk studie av romanen Imaging modaliteter for diagnostisering av lymfeknuter metastaser.
