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JoVE Journal Developmental Biology
An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
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An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

Article DOI: 10.3791/59342-v • 06:36 min • May 13th, 2019
May 13th, 2019 •
1, 1
1Department of Biomedical Engineering, University of Texas at Austin

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Summary
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Endothelial progenitors derived from induced pluripotent stem cells (iPSC-EPs) have the potential to revolutionize cardiovascular disease treatments and to enable the creation of more faithful cardiovascular disease models. Herein, the encapsulation of iPSC-EPs in three-dimensional (3D) collagen microenvironments and a quantitative analysis of these cells’ vasculogenic potential are described.

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In Vitro 3D Model Computational Pipeline Quantify Vasculogenic Potential IPSC-Derived Endothelial Progenitors Molecular Mechanisms Tissue Level Mechanisms Engineering Functional Vasculature Cardiovascular Disease Therapy Robust Vascular Networks Vasculogenic Potential Evaluation Computational Pipeline Analysis Cell Detachment Solution Differentiated Cell Culture P1000 Pipette Tip Single Cell Suspensions Centrifugation Sorting Buffer Fluorescence Conjugated CD34 Antibody Wash Cells Fluorescence Activated Cell Sorting (FACS) High Fluorescence Intensity
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