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Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
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1Stream Biofilm and Ecosystem Research Laboratory, School of Architecture, Civil and Environmental Engineering,Ecole Polytechnique Fédérale de Lausanne, 2Plateforme technique, School of Architecture, Civil and Environmental Engineering,Ecole Polytechnique Fédérale de Lausanne, 3Water Chemistry and Water Technology, Engler-Bunte-Institut,Karlsruhe Institute of Technology, 4Thorlabs GmbH
Chapters
- 00:04Title
- 01:23Overview of Experimental Setup
- 01:41Setup of the Positioning Device
- 02:15Optical Coherence Tomography Setup
- 02:52Image Acquisition
- 06:08Image Correction and Display
- 08:14Results: Effect of Flow Velocity on Biofilm Growth
- 09:10Conclusion
Microbial biofilms form complex architectures at interphases and develop into highly scale-dependent spatial patterns. Here, we introduce an experimental system (hard- and software) for the automated acquisition of 3D optical coherence tomography (OCT) datasets. This toolset allows the non-invasive and multi-scale characterization of biofilm morphogenesis in space and time.
Tags
Keywords:
3D Optical Coherence TomographyBiofilm MorphogenesisAutomated SystemLarge Spatial ScalesExtended Time PeriodsEcosystem ProcessesNatural SystemsEngineered EnvironmentsPrecision Positioning DevicePlexiglass FlumeGRBL ServerMicrofluidic DevicesFlow ChambersFlumesFiltration SystemsOCT SystemImage AcquisitionPositioning DeviceDovetail HolderImmersion Adapter
