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Genetics

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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
 

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Article doi: 10.3791/59382
March 31st, 2019

Summary March 31st, 2019

A CRISPR/sgRNA library has been applied to interrogating protein-coding genes.However, the feasibility of a sgRNA library to uncover the function of a CTCF boundary in gene regulation remains unexplored. Here, we describe a HOX loci specific sgRNA library to elucidate the function of CTCF boundaries in HOX loci.

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