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JoVE Journal
Biochemistry

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In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
 

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

Article DOI: 10.3791/59385
July 25th, 2019

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Summary July 25th, 2019

Ubiquitination is a post-translational modification that plays important roles in cellular processes and is tightly coordinated by deubiquitination. Defects in both reactions underlie human pathologies. We provide protocols for conducting ubiquitination and deubiquitination reaction in vitro using purified components.

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