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JoVE Journal
Biochemistry

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Nitropeptide Profiling and Identification Illustrated by Angiotensin II
 

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Article DOI: 10.3791/59391
June 16th, 2019

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Summary June 16th, 2019

Proteomic profiling of tyrosine-nitrated proteins has been a challenging technique due to the low abundance of the 3-nitrotyrosine modification. Here we describe a novel approach for nitropeptide enrichment and profiling by using Angiotensin II as the model. This method can be extended for other in vitro or in vivo systems.

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