Journal
/
Genetics
/
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

DOI:
10.3791/59394-v

•

09:31 min

•

April 19, 2019

•

, , , , , , ,
1Department of Developmental Physiology,Centre for Organismal Studies (COS) Heidelberg, 2Department of Cell Biology,Centre for Organismal Studies (COS) Heidelberg, 3Department of Stem Cell Biology,Centre for Organismal Studies (COS) Heidelberg

Chapters

  • 00:04Title
  • 00:53Induction of Trans-activation in Arabidopsis Driver Lines: Root
  • 01:49Induction of Trans-activation in Arabidopsis Driver Lines: Stem
  • 02:54Induction of Trans-activation in Arabidopsis Driver Lines: Shoot Apical Meristem (SAM)
  • 03:35Imaging of Reporter Expression in Arabidopsis Driver Lines: Root
  • 04:28Imaging of Reporter Expression in Arabidopsis Driver Lines: Stem
  • 06:02Imaging of Reporter Expression in Arabidopsis Driver Lines: Shoot Apical Meristem
  • 07:41Results: Analysis of Cell Type-Specific Expression in Arabidopsis thaliana
  • 08:38Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe the generation and application of a set of transgenic Arabidopsis thaliana lines enabling inducible, tissue-specific expression in the three main meristems, the shoot apical meristem, the root apical meristem, and the cambium.

Tags

Arabidopsis ThalianaInducible ExpressionCell Type-specificLhGR-mediated Trans-activationGreen Gate CloningMeristemsConfocal MicroscopyDEX InductionSilwet L-77Shoot Apical Meristem

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Controllable Ion Channel Expression through Inducible Transient Transfection

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Read Article