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An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells

Article DOI: 10.3791/59419-v • 04:37 min • December 16th, 2019
December 16th, 2019 •
1,2, 3, 1, 3, 1,2
1Department of Medicine, Division of Hematology/Oncology, New Jersey Medical School, Rutgers Biomedical and Health Sciences, 2Rutgers School of Graduate Studies at New Jersey Medical School, Rutgers Biomedical and Health Sciences, 3Department of Surgery, Division of Plastic Surgery, New Jersey Medical School, Rutgers Biomedical and Health Sciences

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Summary
Automatic Translation

This protocol provides an enzyme-free method for isolating mesenchymal stem cells from abdominoplasty and lipoaspirate samples using an explant method. The absence of harsh enzymes or centrifugation steps provides for clinically relevant stem cells that can be used for studies in vitro or transferred back to the clinic.

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Enzyme-free Method Isolation Expansion Human Adipose-derived Mesenchymal Stem Cells ASCs Cell Phenotype Behavior Experimental Observations Artifact Explant Method Clinical Relevance Diseases Research Applications Mincing Method Tissue Samples Sterile Needle Sterile Scalpel Sterile Spatula Vacuum Gas Plasma Treated Tissue Culture Plate
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