Journal
/
Environment
/
Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals
JoVE Journal
Environment
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals

Using Deuterium Oxide as a Non-Invasive, Non-Lethal Tool for Assessing Body Composition and Water Consumption in Mammals

DOI:
10.3791/59442-v

•

08:22 min

•

February 20, 2020

•

, , , , ,
1Department of Veterinary Pathobiology,University of Missouri, 2College of Veterinary Medicine,University of Missouri, 3Department of Animal Science,University of Missouri, 4School of Natural Resources,University of Missouri, 5USDA USFS Northern Research Station

Chapters

  • 00:05Introduction
  • 01:07Sterile, Isotonic, Salinated Deuterium Oxide (D2O) Preparation
  • 02:38Big Brown Bat (Eptesicus fucsus) Body Composition Determination
  • 04:34Fourier-transform Infrared (FTIR) Spectrophotometry Analysis
  • 06:20Results: Representative D2O Body Composition Assessment Validation
  • 07:38Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes the deuterium oxide dilution technique in two mammals, an insectivore and carnivore, to determine total body water, lean body mass, body fat mass, and water consumption.

Tags

Keywords: Deuterium OxideD2ONon-invasiveNon-lethalBody CompositionWater ConsumptionMammalsStable IsotopeWaterEndangered SpeciesHumansDomestic SpeciesDeuterium Oxide DilutionFree-ranging WildlifeRecapture RatesSocially Housed AnimalsAnesthesiaSubcutaneous InjectionsStock SolutionSodium ChlorideSterileVacuumIsosmotic StrengthInterfemoral VeinPetroleum Jelly29-gauge Needle

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59442?article_type=v

Laboratory-determined Phosphorus Flux from Lake Sediments as a Measure of Internal Phosphorus Loading
check_url/59442?article_type=v

Use of Chironomidae (Diptera) Surface-Floating Pupal Exuviae as a Rapid Bioassessment Protocol for Water Bodies
check_url/59442?article_type=v

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
check_url/59442?article_type=v

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability
check_url/59442?article_type=v

Soil Lysimeter Excavation for Coupled Hydrological, Geochemical, and Microbiological Investigations
check_url/59442?article_type=v

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays
check_url/59442?article_type=v

Protocol for Assessing the Relative Effects of Environment and Genetics on Antler and Body Growth for a Long-lived Cervid
check_url/59442?article_type=v

Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas
check_url/59442?article_type=v

A Simple Planting Technique for Re-establishing Trees Where Frequent Inundation Occurs
check_url/59442?article_type=v

Optimized Procedure for Determining the Adsorption of Phosphonates onto Granular Ferric Hydroxide using a Miniaturized Phosphorus Determination Method

Read Article