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Biochemistry

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JoVE Journal Biochemistry
Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity
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Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity

Article DOI: 10.3791/59452-v • 08:00 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1,2, 1, 1,3, 1, 1, 1, 2, 1, 1
1Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham, 2Division of Cardiovascular Diseases, School of Medicine, School of Engineering, University of Alabama at Birmingham, 3Department of Cardiac Surgery, The Second Xiangya Hospital of Central South University

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Summary
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In this protocol, we demonstrate and elaborate on how to use human induced pluripotent stem cells for cardiomyocyte differentiation and purification, and further, on how to improve its transplantation efficiency with Rho-associated protein kinase inhibitor pretreatment in a mouse myocardial infarction model.

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Engraftment Human Induced Pluripotent Stem Cells Cardiomyocytes Rho Kinase Activity Inhibition Adhesion Survival Transplantation Infarcted Animal Hearts Low-cost Method Cell Engraftment Rates Acute Cardiac Infarction Heart Failure Cardiac Function Vascularities Apoptosis Protocols Y-27632 Treatment Embryonic Stem Cells Mesenchymal Stem Cells Cell-dissociation Enzymes Differentiation
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