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JoVE Journal
Immunology and Infection

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Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems
 

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems

Article doi: 10.3791/59453
April 26th, 2019

Summary April 26th, 2019

In this article, we present two complementary methods to measure specific rates of translational error and mistranslation in the model mycobacterium, Mycobacterium smegmatis, using gain-of-function reporter systems. The methods can be employed to measure accurate error rates in low throughput or relative error rates in a more high-throughput setting.

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