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Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems
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Chapters
- 00:04Title
- 00:52Renilla-firefly Dual-luciferase Reporter
- 03:02Nluc/GFP Reporter
- 05:08Results: Measuring Rates of Translational Error Using Gain-of-function Reporter Systems
- 05:50Conclusion
In this article, we present two complementary methods to measure specific rates of translational error and mistranslation in the model mycobacterium, Mycobacterium smegmatis, using gain-of-function reporter systems. The methods can be employed to measure accurate error rates in low throughput or relative error rates in a more high-throughput setting.
