Journal
/
Bioengineering
/
A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters

DOI:
10.3791/59462-v

•

12:14 min

•

June 13, 2019

•

, , , , , , ,
1Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology,Nanjing Agricultural University, 2College of Life Science,Nanjing Agricultural University

Chapters

  • 00:04Title
  • 01:10RNA Isolation by Guanidinium Thiocyanate-phenol Extraction and cDNA Library Generation by Reverse Transcription
  • 04:35Mitochondrial Gene Amplification, Purification, Sequencing and Comparison
  • 07:27Applying the cDNA Library for Cloning the Genes MgUGD and MgUXS
  • 08:42Expression and Activity Tests of MgUGD and MgUXS
  • 10:32Results: Sequencing and Mass Spectrometry Results
  • 11:33Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a strategy for how to use RNA samples from unreferenced Pacific oyster specimens, and evaluate the genetic material by comparison with publicly available genome data to generate a virtually sequenced cDNA library.

Tags

Keywords: CDNA LibraryPacific OystersRNA ExtractionCytochrome C OxidaseNADH DehydrogenaseBiotechnologyGene Cloning

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59462?article_type=v

Synthetic Spider Silk Production on a Laboratory Scale
check_url/59462?article_type=v

Designing a Bio-responsive Robot from DNA Origami
check_url/59462?article_type=v

An Improved Method for the Preparation of Type I Collagen From Skin
check_url/59462?article_type=v

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
check_url/59462?article_type=v

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
check_url/59462?article_type=v

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry
check_url/59462?article_type=v

A Microcontroller Operated Device for the Generation of Liquid Extracts from Conventional Cigarette Smoke and Electronic Cigarette Aerosol
check_url/59462?article_type=v

Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining
check_url/59462?article_type=v

Production of Membrane-Filtered Phase-Shift Decafluorobutane Nanodroplets from Preformed Microbubbles
check_url/59462?article_type=v

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

Read Article