9,420 Views
•
12:26 min
•
June 17, 2019
DOI:
Summary
Automatically generated
يهدف هذا البروتوكول إلى توليد الخلايا العصبية البينية المعاد برمجتها مباشرة في الجسم الحي، وذلك باستخدام نظام فيروسي قائم على AAV في الدماغ ومراسل GFP يعمل على إزالة المشبك المرن، والذي يسمح بتحديد الخلايا والمزيد من التحليل في الجسم الحي.
Related Videos
08:47
Intracranial Injection of Adeno-associated Viral Vectors
Related Videos
47515 Views
15:00
Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies
Related Videos
5691 Views
19:45
Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection
Related Videos
16863 Views
09:36
Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
Related Videos
12285 Views
10:15
Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction
Related Videos
59329 Views
07:57
Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord
Related Videos
8573 Views
00:06
Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains
Related Videos
5949 Views
11:42
In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
Related Videos
4927 Views
06:12
Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain
Related Videos
3087 Views
07:25
Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes
Related Videos
2749 Views
Read Article
Cite this Article
Pereira, M., Birtele, M., Rylander Ottosson, D. In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors. J. Vis. Exp. (148), e59465, doi:10.3791/59465 (2019).
Copy