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Journal / Neuroscience / In vivo directe herprogrammering van ingezeten…
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Neuroscience

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JoVE Journal Neuroscience
In vivo directe herprogrammering van ingezeten gliacellen in interneuronen door intracerebrale injectie van virale vectoren
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In vivo directe herprogrammering van ingezeten gliacellen in interneuronen door intracerebrale injectie van virale vectoren

Article DOI: 10.3791/59465-v • 12:26 min • June 17th, 2019
June 17th, 2019 •
1, 1, 1
1Department of Experimental Medical Sciences and Lund Stem Cell Center, BMC, Lund University

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Dit protocol is gericht op het genereren van direct geherprogrammeerde interneuronen in vivo, met behulp van een AAV-gebaseerd virale systeem in de hersenen en een FLEX synapsin-driven GFP Reporter, die het mogelijk maakt voor celidentificatie en verdere analyse in vivo.

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Neuroscience directe conversie whole-Cell Patch clamp FLEX vector systeem CRE-inducible systeem slice-opnames parvalbumin trans differentiatie neurale conversie
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