Journal
/
Chemistry
/
Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue

Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue

DOI:
10.3791/59471-v

•

07:09 min

•

May 09, 2019

•

, , , , ,
1Department of Biological and Environmental Sciences,Longwood University, 2Department of Chemistry and Physics,Longwood University

Chapters

  • 00:04Title
  • 00:47Restriction Enzyme Digestion
  • 01:50Ligation
  • 02:29Colony PCR
  • 03:14Titrating E. coli to Check Function of Device
  • 03:54Using E. coli as Chemical Sensor for GSR
  • 04:44Fluorescence Analysis Using Portable Spectrometer
  • 05:09Fluorescence Analysis Using 96-well Plate Reader
  • 05:34Results: Analysis of the Biosensor and Presumptive Detection of Gunshot Residue
  • 06:29Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol is presented using synthetic biology techniques to synthesize a set of bacterial biosensors for the analysis of gunshot residue, and to test the functioning of the devices for their intended use using fluorescence spectroscopy.

Tags

Keywords: Bacterial BiosensorGunshot ResidueForensic ApplicationsSynthetic BiologyPlasmid DNARestriction EnzymesDNA LigationBacterial TransformationPCR

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59471?article_type=v

Preparation and Use of Samarium Diiodide (SmI2) in Organic Synthesis: The Mechanistic Role of HMPA and Ni(II) Salts in the Samarium Barbier Reaction
check_url/59471?article_type=v

Synthesis of Hypervalent Iodonium Alkynyl Triflates for the Application of Generating Cyanocarbenes
check_url/59471?article_type=v

Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography
check_url/59471?article_type=v

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation
check_url/59471?article_type=v

Protocol for the Synthesis of Ortho-trifluoromethoxylated Aniline Derivatives
check_url/59471?article_type=v

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
check_url/59471?article_type=v

Facile Synthesis of Worm-like Micelles by Visible Light Mediated Dispersion Polymerization Using Photoredox Catalyst
check_url/59471?article_type=v

Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
check_url/59471?article_type=v

Total Internal Reflection Absorption Spectroscopy (TIRAS) for the Detection of Solvated Electrons at a Plasma-liquid Interface
check_url/59471?article_type=v

Color Spot Test As a Presumptive Tool for the Rapid Detection of Synthetic Cathinones

Read Article