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High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
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Bioengineering
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JoVE Journal Bioengineering
High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

DOI:
10.3791/59474-v

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09:20 min

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June 02, 2019

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, ,
1BioSystems and Micromechanics Interdisciplinary Research Group,Singapore-MIT Alliance for Research and Technology, CREATE, 2Department of Material Science and Engineering,Massachusetts Institute of Technology, 3Department of Biological Engineering,Massachusetts Institute of Technology

Chapters

  • 00:04Title
  • 00:37Fabrication of Single-well Polydimethylsiloxane Plates and Square Compartments
  • 02:52Cell Culture
  • 04:28Imaging
  • 07:14Results: Fluctuations Data of the Nucleus in Response to Chemical and Strain Cues
  • 08:31Conclusion

Summary

Automatic Translation

Automatic Translation

Using a previously designed device to apply mechanical strain to adherent cells, this paper describes a redesigned substratum geometry and a customized apparatus for high-resolution single-cell imaging of strained cells with a 100x oil immersion objective.

Tags

Keywords: High-resolution ImagingNuclear DynamicsLive CellsUniaxial Tensile StrainCell-stretching SystemPolydimethylsiloxaneSubstratum FabricationProgenitor CellsProliferation Medium

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