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Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry
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Cancer Research
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JoVE Journal Cancer Research
Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry

Capturing Small Molecule Communication Between Tissues and Cells Using Imaging Mass Spectrometry

DOI:
10.3791/59490-v

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07:58 min

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April 03, 2019

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, , ,
1Department of Medicinal Chemistry and Pharmacognosy,University of Illinois at Chicago, 2Department of Animal Science,University of Illinois at Urbana-Champaign

Chapters

  • 00:04Title
  • 01:16Setting Up and Incubating the ITO (Indium Tinoxide)-treated Slide for Cocultures
  • 04:04Drying Slide and Preparing for MALDI-TOF MS (Matrix-assisted Laser Desorption/Ionization Mass Spectrometry)
  • 05:48Results: Proper Sample Preparation and Representative IMS Data
  • 07:16Conclusion

Summary

Automatic Translation

Automatic Translation

A novel method of sample preparation was developed to accommodate cell and tissue coculture to detect small molecule exchange using imaging mass spectrometry.

Tags

Keywords: Small MoleculesImaging Mass SpectrometryOvarian CancerMetastasisSpatial AnalysisCell-cell CommunicationAgaroseCell CultureCo-cultureLabel-freeTherapeutic Targets

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