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Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells
 

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

Article DOI: 10.3791/59491-v
November 26th, 2019

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Summary November 26th, 2019

Here, we present a protocol for imaging and quantifying calcium dynamics in heterogeneous cell populations, such as pancreatic islet cells. Fluorescent reporters are delivered into the peripheral layer of cells within the islet, which is then immobilized and imaged, and per-cell analysis of the dynamics of fluorescence intensity is performed.

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