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Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells
JoVE Journal
Biology
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JoVE Journal Biology
Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

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08:03 min

November 26, 2019

DOI:

08:03 min
November 26, 2019

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Summary

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Here, we present a protocol for imaging and quantifying calcium dynamics in heterogeneous cell populations, such as pancreatic islet cells. Fluorescent reporters are delivered into the peripheral layer of cells within the islet, which is then immobilized and imaged, and per-cell analysis of the dynamics of fluorescence intensity is performed.

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