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JoVE Journal
Genetics

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Complexos artificiais do alongamento do polymerase II do RNA para dissecando eventos co-transcriptional do processamento do RNA
 
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Complexos artificiais do alongamento do polymerase II do RNA para dissecando eventos co-transcriptional do processamento do RNA

Article DOI: 10.3791/59497
May 13th, 2019

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Summary May 13th, 2019

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Aqui, nós descrevemos o conjunto de complexos do alongamento do polymerase II (pol II) do RNA que exigem somente o ADN sintético curto e oligonucleotídeos do RNA e o pol purificado II. Esses complexos são úteis para estudar mecanismos subjacentes ao processamento cotranscricional de transcrições associadas ao complexo de alongamento pol II.

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