Name: Identification and Dissection of Diverse Mouse Adipose Depots (Video) | JoVE Journal
Uploaded: 2019-07-11T12:30:00.000+00:00
Duration: 6 min 31 s
Description: Learn standardized dissection techniques to identify and isolate diverse mouse adipose depots including brown, subcutaneous, visceral, and popliteal tissues. This protocol enables reproducible downstream histological and molecular analyses.

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5 minutes

O.A.M. is supported by NIH grants DK062876 and DK092759; D.P.B. is supported by the University of Michigan Medical Scientist Training Program (T32GM007863), University of Michigan Training Program in Organogenesis (T32HD007605), University of Michigan Rackham Merit Fellowship, and Tylenol Future Care Fellowship.

All animal procedures are performed with the approval of the Institutional Animal Care and Use Committee (IACUC) of the University of Michigan.
1. Euthanization
NOTE: For the purpose of this video protocol, 4 to 6 month-old C57BL/6J mice are used.
<ol>
	<li>Place the mouse in an isoflurane vaporizer chamber and adjust the isoflurane flow rate to 5% or greater. Continue isoflurane exposure until one minute after breathing stops. Then remove the mouse from the vaporizer chamber and confirm euthanasia using an approved secondary measure. 
	NOTE: Approved secondary measures will vary by institution and animal protocol and may include cervical dislocation or decapitation.</li>
	<li>Place the euthanized mouse on the dissection pan. Sterilize the dorsal and ventral external surfaces of the mouse using 70% ethanol. Ensure that the external surface of the mouse is sufficiently wet to minimize contamination from fur during dissection.</li>
</ol>
2. Identification and Isolation of Major Subcutaneous Adipose Ddepots (Anterior Subcutaneous, Dorsolumbar, Inguinal, Gluteal) and Interscapular Brown Aadipose Tissue
<ol>
	<li>Identifying and isolating anterior subcutaneous WAT 
	NOTE: Anterior subcutaneous WAT is located between the scapulae, descending from the nape of the neck to the axillae of the mouse7. This depot has alternatively been described as suprascapular8 or interscapular20 WAT and lies directly on top of the interscapular BAT depot.
	<ol>
		<li>To isolate the anterior subcutaneous depot, lay the mouse on its stomach in a prone position. Secure the upper and lower limbs to the dissection pan with dissection pins.</li>
		<li>Use forceps to lift the dorsal skin at the nape of the neck. Use iris scissors to make a small (1 mm) cut in the skin.</li>
		<li>Insert one blade of the iris scissors into the initial cut and make a midline vertical incision (2&#8211;3 cm) through the skin, beginning at the nape of the neck and descending along the spine to mid-back.</li>
		<li>Make two horizontal incisions (1 cm each) using the iris scissors, extending laterally from midline, at the top and bottom of the initial vertical incision.</li>
		<li>Use forceps to carefully peel back the skin and expose the anterior subcutaneous depot.</li>
		<li>Use iris scissors to remove the depot following the natural borders of the tissue. 
		NOTE: This method isolates both anterior subcutaneous WAT and interscapular BAT.</li>
		<li>Place the dissected depot on the dissection pan and carefully remove the contaminating BAT using iris scissors.</li>
	</ol>
	</li>
	<li>Identifying and isolating classical BAT 
	NOTE: Classical BAT is located beneath the anterior subcutaneous WAT depot.
	<ol>
		<li>To isolate BAT, use iris scissors to cut horizontally along the bottom edge of the anterior subcutaneous tissue, following the natural border of the depot.</li>
		<li>Then, use iris scissors to make two vertical incisions along the lateral edges of the depot, following the natural borders of the tissue.</li>
		<li>Use forceps to carefully flip the depot up and reveal the butterfly-shaped interscapular BAT embedded within the WAT. Carefully dissect the BAT out from the surrounding WAT.</li>
	</ol>
	</li>
	<li>Identifying and isolating posterior subcutaneous WAT,
	<ol>
		<li>Lay the mouse on its back in a supine position.</li>
		<li>After securing the upper and lower limbs with dissection pins, use forceps to lift up the skin at the base of the sternum and make a small (1 mm) cut in the skin.</li>
		<li>Insert one blade of the iris scissors into the initial cut and make a midline incision (4&#173;&#8211;5 cm) through the skin, beginning at the base of the sternum and descending to the base of the tail. Exercise caution when making this incision because the ventral skin is very thin and is closely associated with the underlying peritoneal cavity wall.</li>
		<li>Make two horizontal incisions (1 cm each) using iris scissors, extending laterally from midline, at the top of the initial vertical incision.</li>
		<li>Use forceps to carefully peel back the skin from the peritoneal cavity and the leg to find posterior subcutaneous WAT, which should remain associated with the skin. Secure the outstretched skin with dissection pins to ease complete excision of the WAT. 
		NOTE: Although the posterior subcutaneous WAT appears to be continuous, it is actually comprised of three discrete depots: dorsolumbar, inguinal, and gluteal1. The dorsolumbar depot extends from the lumbar spine to the base of the hindlimb. The triangular inguinal depot extends from the base of the hindlimb ventrally across the groin and contains a prominent lymph node. The gluteal depot extends inferiorly from the base of the groin and wraps around the leg to the base of the tail.</li>
	</ol>
	</li>
</ol>
3. Identification and Isolation of Visceral Adipose Depots (Gonadal, Perirenal, Retroperitoneal, Omental, Pericardial)
<ol>
	<li>Identifying and isolating visceral WAT depots 
	NOTE: WAT depots, which are largely contained within the thoracic and peritoneal cavities, keep the mouse in a supine position. Secure the upper and lower limbs to the dissection pan with dissection pins.
	<ol>
		<li>Use forceps to lift up the thin peritoneal cavity wall at the base of the sternum and make a small cut (1 mm) using iris scissors.</li>
		<li>Insert one blade of the iris scissors into the cut and make a descending vertical incision (4&#8211;5 cm) from the top of the peritoneal cavity (base of the sternum) to the rectum.</li>
		<li>Make two horizontal incisions (1 cm each) with iris scissors, extending laterally from midline, at the top and bottom of the vertical incision.</li>
		<li>Use forceps to peel back the peritoneum and expose the abdominal cavity contents. Pin the outstretched peritoneum to the dissection pan.</li>
	</ol>
	</li>
	<li>Identifying and isolating gonadal WAT 
	NOTE: Gonadal WAT surrounds the uterus and ovaries in females (ovarian or parametrial) and the epididymis and testes in males (epididymal). Ovarian WAT surrounds the ovaries, uterus, and bladder. In obese animals, gonadal and perirenal WAT can appear to be continuous &#8212; in this case, separate the depots at the uterine horn and ovaries. Epididymal WAT is found bound to the epididymis, testes, and the prominent epididymal blood vessel.
	<ol>
		<li>Locate the gonads (testes or ovaries) and use forceps to lift up the associated gonadal WAT.</li>
		<li>Use iris scissors to carefully excise the WAT from the gonads.</li>
	</ol>
	</li>
	<li>Identifying and isolating perirenal WAT 
	NOTE: Perirenal WAT surrounds the kidneys bilaterally. In obese animals, this depot can appear to extend inferiorly to the top of the uterine horn and ovaries. Although the perirenal WAT has traditionally been classified as a visceral depot21, several groups have identified it as a brown depot based on lineage tracing and radiolabeled glucose uptake studies8,9,20. Histologically it is comprised of a mixture of white and brown adipocytes.
	<ol>
		<li>To excise the perirenal depot, locate the kidney and use forceps to lift it up and pull it midline to see a clear division between the perirenal and retroperitoneal depots.</li>
		<li>Excise the WAT directly associated with the kidneys. Ensure that the adrenal glands, located above the kidneys, are removed from the WAT.</li>
	</ol>
	</li>
	<li>Identifying and isolating retroperitoneal WAT 
	NOTE: Retroperitoneal WAT is located in a paravertebral position, along the border between the posterior abdominal wall and the spinal cord.
	<ol>
		<li>To excise this depot, use forceps to lift the kidney up and towards midline to clearly see the border between the perirenal and retroperitoneal depots. 
		NOTE: In obese animals, identifying this border can be challenging.</li>
		<li>Then, use iris scissors to carefully dissect the retroperitoneal WAT from the posterior peritoneal wall.</li>
	</ol>
	</li>
	<li>Identifying and isolating omental WAT 
	NOTE: Omental WAT is located along the greater curvature of the stomach. Although omental adipose is an important depot in humans, it is generally present only in morbidly obese mice.
	<ol>
		<li>To identify visceral omental WAT, use forceps to lift the stomach up. Use iris scissors to remove the associated adipose tissue along the inferior border of the stomach. Do not confuse omental WAT with the pancreas.</li>
	</ol>
	</li>
	<li>Identifying and isolating mesenteric WAT 
	NOTE: Mesenteric WAT is a web-like structure surrounding and associated with the small and large intestines.
	<ol>
		<li>To excise this depot, remove the intestines from the rest of the digestive tract by cutting at the base of the stomach and the rectum. Use forceps to lift the intestines out of the visceral cavity and unravel them.</li>
		<li>Use iris scissors to carefully dissect the mesenteric WAT away from the intestines, beginning at the duodenum and continuing to the end of the colon. Carefully remove lymph nodes that are closely associated with the mesenteric depot.</li>
	</ol>
	</li>
	<li>Identifying and isolating pericardial WAT 
	NOTE: Pericardial WAT is located outside the visceral pericardium and on the external surface of the parietal pericardium, often along the inferior aspect of the heart14.
	<ol>
		<li>To gain access to the thoracic cavity, keep the mouse in a supine position. Secure the upper and lower limbs to the dissection pan with dissection pins.</li>
		<li>Use forceps to lift the xyphoid process (cartilage at the base of the sternum) and make a small (1 mm) cut in the thoracic cavity wall.</li>
		<li>Insert one blade of the iris scissors into the cut and make two horizontal incisions (1 cm each) extending laterally from the base of the sternum. This will expose the diaphragm and inferior border of the thoracic cavity.</li>
		<li>Use dissecting scissors to make two ascending vertical incisions (3&#8211;4 cm) through the ribcage, extending superiorly from the lateral borders of the thoracic cavity to the clavicle.</li>
		<li>Use forceps to lift up the ventral half of the ribcage. Use dissecting scissors to make a final cut (2 cm) along the inferior length of the clavicle to remove the ribcage and expose the thoracic cavity contents.</li>
		<li>If present, carefully dissect the pericardial adipose from the outer surface of the pericardium.</li>
	</ol>
	</li>
</ol>
4. Identification and Isolation of Other Adipose Depots
<ol>
	<li>Identifying and isolating epicardial WAT 
	NOTE: Epicardial WAT is contained within the visceral pericardium and is directly associated with the surface of the myocardium.
	<ol>
		<li>If present, epicardial adipocytes can be observed histologically after isolation and fixation of the perfused heart in 10% neutral buffered formalin for 24 h.</li>
	</ol>
	</li>
	<li>Identifying and isolating popliteal WAT 
	NOTE: Popliteal WAT is located in the popliteal fossa in the posterior knee and contains a large lymph node. This depot is not typically visible in young animals.
	<ol>
		<li>To isolate popliteal WAT, use iris scissors to carefully remove the skin from the base of the hind limb to the foot.</li>
		<li>Place the patella against the dissection pan and secure the outstretched leg with a pin in the foot. Ensure that the popliteal fossa at the back of the knee is facing upward.</li>
		<li>Use iris scissors to make a cut at the inferior border of the medial and lateral heads of the gastrocnemius muscle. Use forceps to lift up the muscle and reveal the triangular popliteal depot.</li>
		<li>Use iris scissors to excise the depot along the natural tissue borders.</li>
	</ol>
	</li>
	<li>Identifying and isolating dermal WAT 
	NOTE: Dermal WAT is a thin layer of adipocytes located between the reticular dermis and the panniculus carnosus muscle layer.
	<ol>
		<li>To identify dermal adipocytes by histological methods, place the mouse on its stomach in a prone position. After securing the upper and lower limbs with dissection pins, spray the external surface of the mouse with 70% ethanol to wet the skin.</li>
		<li>Use a scalpel to remove the wetted fur from a square portion of skin on the back of the mouse.</li>
		<li>Use iris scissors to carefully excise the shaved portion of skin, which will include the reticular dermis, dermal WAT, panniculus carnosus, and some subcutaneous WAT.</li>
		<li>Use a scalpel to cut the excised skin into thin vertical strips.</li>
		<li>Position the thin vertical strips with the sticky subcutaneous WAT layer facing down.</li>
		<li>Starting at one end, roll the strip onto itself to form a spiral. The sticky subcutaneous WAT layer on the outside of the spiral will allow the roll to maintain its shape during fixation.</li>
		<li>Place the spiral in a well of a 24 well plate containing 10% neutral buffered formalin for 24 h prior to histological processing22.</li>
	</ol>
	</li>
	<li>Identifying and isolating intermuscular WAT 
	NOTE: Intermuscular WAT is broadly defined as adipocytes located beneath the deep fascia of muscles. This term includes adipocytes interspersed between muscle fibers of skeletal muscle, also known as intramuscular WAT, and adipocytes located within muscle bundles themselves. Wildtype mice generally do not have large numbers of intermuscular adipocytes. However, it is possible under certain conditions to identify intermuscular WAT by histological methods. Under some conditions, intermuscular adipocytes can also be found in smooth muscles, such as the diaphragm.
	<ol>
		<li>To isolate the tibialis anterior (TA) muscle, for example, place the mouse on its back in a supine position and secure the lower limbs to the dissection pan using dissection pins. Wet the skin with 70% ethanol to minimize contamination with fur.</li>
		<li>Use iris scissors to carefully remove the skin from the leg and expose the quadriceps muscle group, located above the knee on the femur shaft, and the TA located below the knee on the ventral surface of the tibia. 
		NOTE: The TA is thick near the proximal end of the tibia and more tendinous near the distal end.</li>
		<li>Use iris scissors to excise a piece of the muscle and fix in 10% neutral buffered formalin for 24 h prior to histological processing22.</li>
	</ol>
	</li>
	<li>Identifying and isolating intra-articular WAT depots 
	NOTE: Intra-articular WAT depots are located within synovial joints.
	<ol>
		<li>To identify infrapatellar WAT, for example, by histological methods, harvest leg bones as detailed in the BMAT section.</li>
		<li>After the removing the femur-tibial complex, use iris scissors and gauze pads to remove as much muscle and connective tissue as possible. Do not break the tibiofemoral joint.</li>
		<li>Fix the femur-tibial complex in 10% neutral buffered formalin for 24 h prior to decalcification and histological processing (described below, step 4.6.8).</li>
	</ol>
	</li>
	<li>Identifying and isolating bone marrow adipose tissue 
	NOTE: Bone marrow adipose tissue (BMAT) is contained within bones, interspersed with hematopoietic cells. Anatomically, BMAT can be classified as constitutive (distal tibia and caudal vertebrae) or regulated (mid-to-proximal tibia, femur, and lumbar vertebrae)10,11. Clean isolation of bone marrow adipocytes for RNA and protein analyses is challenging in mice. However, mouse bones can readily be harvested, fixed, decalcified and paraffin-embedded for histological analyses.
	<ol>
		<li>To harvest leg bones, for example, first remove the legs from the mouse. Use dissection scissors to cut the acetabulofemoral joint, keeping the femoral head intact.</li>
		<li>Use iris scissors to carefully remove the skin from the leg, revealing the leg muscles.</li>
		<li>Carefully dissect away the main muscles from the femur and tibia using iris scissors and gauze pads.</li>
		<li>When the femur is exposed, follow the edge of the bone to the articulation of the pelvis and femur and carefully release the femoral head from the acetabulofemoral joint. Use gauze to clean any remaining tissue from the femur.</li>
		<li>Follow the border of the tibia to the ankle joint. Carefully release the medial malleolus, located at the tip of the tibia, from the ankle joint.</li>
		<li>Once the femur-tibial complex has been isolated, remove as much muscle and connective tissue as possible using iris scissors and/or gauze.</li>
		<li>Separate the tibia from the femur by inserting one blade of the iris scissors into the tibiofemoral joint and gently cut through the medial and lateral collateral ligaments and the anterior and posterior cruciate ligaments. Do not remove the capsule of the knee joint to ensure that the tibia remains intact. Use gauze to clean any remaining tissue from the tibia.</li>
		<li>Fix bones in 10% neutral buffered formalin for 24 h at room temperature and then wash and store according to future needs.
		<ol>
			<li>To analyze bone parameters using microcomputed tomography (&#181;CT), store bones in Sorensen&#8217;s phosphate buffer, pH 7.423.</li>
			<li>For BMAT quantification and histological analyses, decalcify bones in 14% EDTA, pH 7.4, for 10-14 days.</li>
			<li>Following decalcification, use osmium tetroxide staining and &#181;CT analysis to quantify BMAT24. Otherwise, process and paraffin-embed bones for histology23.</li>
		</ol>
		</li>
	</ol>
	</li>
</ol>

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<li>Scheller, E. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Region-specific+variation+in+the+properties+of+skeletal+adipocytes+reveals+regulated+and+constitutive+marrow+adipose+tissues%5BTitle%5D)+AND+%22Nature+Communications%22%5BJournal%5D)">Region-specific variation in the properties of skeletal adipocytes reveals regulated and constitutive marrow adipose tissues.</a> Nature Communications. 6, 7808(2015).</li>
<li>Scheller, E. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Use+of+osmium+tetroxide+staining+with+microcomputerized+tomography+to+visualize+and+quantify+bone+marrow+adipose+tissue+in+vivo%5BTitle%5D)+AND+%22Methods+in+Enzymology%22%5BJournal%5D)">Use of osmium tetroxide staining with microcomputerized tomography to visualize and quantify bone marrow adipose tissue in vivo.</a> Methods in Enzymology. 537, 123-139 (2014).</li>
<li>Lukjanenko, L., Brachat, S., Pierrel, E., Lach-Trifilieff, E., Feige, J. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Genomic+profiling+reveals+that+transient+adipogenic+activation+is+a+hallmark+of+mouse+models+of+skeletal+muscle+regeneration%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Genomic profiling reveals that transient adipogenic activation is a hallmark of mouse models of skeletal muscle regeneration.</a> PLoS One. 8 (8), e71084(2013).</li>
<li>Pagano, A. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Muscle+Regeneration+with+Intermuscular+Adipose+Tissue+%28IMAT%29+Accumulation+Is+Modulated+by+Mechanical+Constraints%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Muscle Regeneration with Intermuscular Adipose Tissue (IMAT) Accumulation Is Modulated by Mechanical Constraints.</a> PLoS One. 10 (12), e0144230(2015).</li>
<li>Khan, I. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intermuscular+and+perimuscular+fat+expansion+in+obesity+correlates+with+skeletal+muscle+T+cell+and+macrophage+infiltration+and+insulin+resistance%5BTitle%5D)+AND+%22International+Journal+of+Obesity%22%5BJournal%5D)">Intermuscular and perimuscular fat expansion in obesity correlates with skeletal muscle T cell and macrophage infiltration and insulin resistance.</a> International Journal of Obesity. 39 (11), 1607-1618 (2015).</li>
<li>Sulston, R. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Increased+Circulating+Adiponectin+in+Response+to+Thiazolidinediones%3A+Investigating+the+Role+of+Bone+Marrow+Adipose+Tissue%5BTitle%5D)+AND+%22Frontiers+in+Endocrinology%22%5BJournal%5D)">Increased Circulating Adiponectin in Response to Thiazolidinediones: Investigating the Role of Bone Marrow Adipose Tissue.</a> Frontiers in Endocrinology. 7, 128(2016).</li>
</ol>

The authors have nothing to disclose.

As the importance of the diverse molecular and functional characteristics of discrete adipocyte clusters is increasingly recognized, it is crucial that investigators within the field uniformly identify and excise adipose depots for further analyses. To date, few protocols exist for standardized localization and isolation of the wide range of mouse adipose depots. Previously published methods are focused primarily on one or two depots and lack the details necessary for uniform identification and excision by different inve...

Adipose tissues play critical roles in whole-body homeostasis, including storage and release of energy in response to local and global needs, thermoregulation, and secretion of adipokines to regulate energy balance, metabolism and immune responses1,2. Adipocytes are distributed throughout the body in discrete depots, and in some cases serve specialized roles within their microenvironments3,4,5. Historically, the study of adipose tissue has centered on white adipose tissue (WAT), and its role in maintaining energy homeostasis. Most adipocytes are distributed throughout the body in subcutaneous and visceral WAT depots. The characteristics of these depots are important for differential susceptibility to metabolic diseases. Subcutaneous adipocytes, located beneath the skin, have been associated with protective metabolic effects5. Visceral adipocytes, which surround the vital organs and are contained within the gonadal, perirenal, retroperitoneal, omental and pericardial depots, are commonly linked to metabolic disorders, including type 2 diabetes and cardiovascular disease2. Brown adipose tissues (BAT) have also been studied extensively. Brown and brown-like adipocytes express uncoupling protein 1 (UCP1) and play important roles in adaptive thermogenesis and glucose homeostasis6,7. Classical brown adipocytes are contained in the interscapular BAT depot8. Clusters of brown adipocytes are also found in other locations, including supraclavicular, infra/subscapular, cervical, paravertebral and periaortic depots8,9.
In addition to their location in major WAT and BAT depots, adipocytes exist in discrete niches throughout the body4, where they may perform specialized functions within their respective microenvironments. For example, bone marrow adipose tissue (BMAT) serves as a lipid reservoir, is a major source of circulating adiponectin, and closely interacts with osteoblasts, osteoclasts, and hematopoietic cells10,11. Dermal adipocytes contribute to widespread processes, including wound healing, immune response, thermoregulation, and hair follicle growth12,13. Further, epicardial adipocytes may produce several adipokines and chemokines that exert local and systemic effects on the development and progression of coronary artery disease14. Expansion of inter/intramuscular WAT has been positively correlated with increased adiposity, systemic insulin resistance, and decreased muscular strength and mobility15. In addition, popliteal adipocytes serve as a lipid reservoir for lymphatic expansion during infection16. While the specific roles of different articular depots are generally unknown, the Hoffa depot (infrapatellar) within the knee is now thought to contribute to pathologies, including anterior knee pain and osteoarthritis17.
Whereas regional differences in adipocyte character and function are under intense study, the field is currently limited by the lack of a standardized protocol for the identification and dissection of diverse mouse depots. Previously published methods have typically described the isolation of one or two specific depots and lack the level of detail required for uniform excision18,19. The protocol described in this manuscript provides a comprehensive guide for the specific anatomic locations and isolation steps of many different mouse adipose depots. Although WAT depots are the primary focus of this manuscript, the excision of interscapular BAT is also described in detail. Adipose tissues excised using this protocol can be used for a wide variety of experimental endpoints, including explant studies, histology, and gene expression analyses.
The goal of this manuscript is to provide investigators with a detailed protocol to clearly and precisely identify and isolate both prominent and less-studied mouse adipose depots (Figure 1). This resource will facilitate a more complete investigation of the developmental, molecular, and functional characteristics of adipocytes within diverse niches.
<img alt="Mouse body fat distribution diagram with labeled adipose tissue, scientific study of obesity." class="xfigimg" src="/files/ftp_upload/59499/59499fig1.jpg" data-altupdatedat="1788282455000" data-alt="Figure 1"> 
Figure 1: Schematic depiction of mouse adipose depots dissected in this protocol. This image has been adapted from Bagchi et al., 20184. <a href="https://www.jove.com/files/ftp_upload/59499/59499fig1large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

<table><tbody><tr><td>10% neutral buffered formalin</td><td>Fisher Scientific</td><td>22-110-869</td><td></td></tr><tr><td>24-well plates, untreated</td><td>Sigma-Aldrich</td><td>CLS3738</td><td></td></tr><tr><td>70% ethanol (dilute from 95%)</td><td>Fisher Scientific</td><td>04-355-226</td><td></td></tr><tr><td>Dissecting forceps with curved tips</td><td>VWR</td><td>89259-946</td><td></td></tr><tr><td>Dissecting pan</td><td>Carolina Biological Supply Company</td><td>629004</td><td></td></tr><tr><td>Dissecting scissors (sharp/blunt tip)</td><td>VWR</td><td>82027-588</td><td></td></tr><tr><td>Gauze sponges</td><td>Vitality Medical</td><td>2634</td><td>Curity 4 inch x 4 inch gauze sponge, 12 ply</td></tr><tr><td>Handi-Pins for dissection</td><td>Carolina Biological Supply Company</td><td>629132</td><td></td></tr><tr><td>Iris scissors (straight)</td><td>VWR</td><td>470018-890</td><td></td></tr><tr><td>Isoflurane</td><td>VetOne</td><td>501017</td><td></td></tr><tr><td>Scalpel</td><td>VWR</td><td>100499-578</td><td>Feather scalpel handle with blade, disposable</td></tr></tbody></table>

identification and dissection of diverse mouse adipose depots

Identification and Dissection of Diverse Mouse Adipose Depots

Adipose tissues are complex organs with a wide array of functions, including storage and mobilization of energy in response to local and global needs, uncoupling of metabolism to generate heat, and secretion of adipokines to regulate whole-body homeostasis and immune responses. Emerging research is identifying important regional differences in the developmental, molecular, and functional profiles of adipocytes located in discrete depots throughout the body. Different properties of the depots are medically relevant since metabolic diseases often demonstrate depot-specific effects. This protocol will provide investigators with a detailed anatomic atlas and dissection guide for the reproducible and accurate identification and excision of diverse mouse adipose tissues. Standardized dissection of discrete adipose depots will allow detailed comparisons of their molecular and metabolic characteristics and contributions to local and systemic pathologic states under various nutritional and environmental conditions.

Successful identification and isolation of various mouse adipose depots can be achieved using the protocol described above. The gross anatomical locations of subcutaneous (A, E-F), brown (B), visceral (C, D, G-J), and popliteal (K) depots are shown in Figure 2.
<img alt="Dissection diagram showing internal organs of a rodent for anatomy study, labeled parts visible." class="xfigimg" src="/files/ftp_...

Adipocytes exist in discrete depots and have diverse roles within their unique microenvironments. As regional differences in adipocyte character and function are uncovered, standardized identification and isolation of depots is crucial for advancement of the field. Herein, we present a detailed protocol for the excision of various mouse adipose depots.

Adipose tissues are complex organs with a wide array of functions, including storage and mobilization of energy in response to local and global needs, ...

identification-and-dissection-of-diverse-mouse-adipose-depots

Research

JoVE Journal

Biology

In this video, we demonstrate the surgical procedure to isolate the subcutaneous white adipose tissue and interscapular brown adipose tissue from a newborn mouse.

White and Brown Adipose Depot Collection from Mouse Pup: A Surgical Procedure to Harvest the White Adipose Tissue and Brown Adipose Tissue from Mouse Pup

Due to the drastic and negative connection between obesity and other comorbidities, research on the role adipose plays in disease and overall health is warranted. We present a protocol for the isolation and excision of adipose depots allowing for the study of adipose using in situ and in vitro methods.

Localization, Identification, and Excision of Murine Adipose Depots

Adipose tissue (AT) can influence whole body homeostasis, therefore understanding the molecular mechanisms of adipocyte differentiation and function is of importance. We provide a protocol for gaining new insights into these processes by analyzing adipocyte homeostasis, differentiation and hypoxia exposure as a model for induced adipocyte apoptosis.

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

The purpose of this paper is to present a step-by-step procedure to collect different white adipose tissues from mice, process the fat samples and extract RNA.

Mouse Adipose Tissue Collection and Processing for RNA Analysis

This protocol describes the technical approach to isolate adipogenic and fibro-inflammatory stromal cell subpopulations from murine intra-abdominal white adipose tissue (WAT) depots by fluorescence-activated cell sorting or immunomagnetic bead separation.

Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging.

Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging

This report describes a protocol for the simultaneous isolation of primary brown and white preadipocytes from newborn mice. Isolated cells can be grown in culture and induced to differentiate into fully mature white and brown adipocytes. The method enables genetic, molecular, and functional characterization of primary fat cells in culture.

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice

This protocol details a method for the dissection of mouse adipose depots and the isolation and digestion of respective arteries to liberate and then identify the endothelial cell population. Freshly isolated cells used in downstream applications will advance the understanding of vascular cell biology and the mechanisms of vascular dysfunction.

Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

Preadipocytes are isolated from the stromal vascular fraction of interscapular brown adipose tissue from newborn mice and differentiated into cells that accumulate lipid droplets, express molecular markers, and show the mitochondrial morphology of mature brown adipocytes. These cells are further analyzed by immunofluorescence and transmission electron microscopy.

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Here, we provide a practical procedure for dissecting and performing histological and gene expression analyses of murine supraclavicular brown adipose tissue.

Dissection, Histological Processing, and Gene Expression Analysis of Murine Supraclavicular Brown Adipose Tissue

The size and fragility of mature adipocytes have limited the techniques and tools available for their study and isolation. Here, a protocol is described for the isolation of mature murine adipocytes, which can be easily adapted for different adipose depots and mouse diets.

Isolation and Culturing of Primary Murine Adipocytes from Lean and Obese Mice

Learn standardized dissection techniques to identify and isolate diverse mouse adipose depots including brown, subcutaneous, visceral, and popliteal tissues. This protocol enables reproducible downstream histological and molecular analyses.

Identification and Dissection of Diverse Mouse Adipose Depots

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