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Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

DOI:
10.3791/59520-v

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09:45 min

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August 08, 2019

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,
1Department of Molecular Biophysics and Biochemistry,Yale University, 2Harvard Medical School,Harvard University

Chapters

  • 00:04Title
  • 00:46Microscope Modification and Chamber Preparation
  • 02:32Microscope Alignment
  • 03:43Control: Imaging Stabilized Microtubules
  • 04:43Imaging Microtubule Dynamics
  • 06:28Properly Setting the Aperture Diaphragm Size
  • 08:11Results: IRM Imaging of Microtubules Dynamics and Contrast Enhancement using Image Processing
  • 09:02Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol is a guide for implementing interference reflection microscopy on a standard fluorescence microscope for label-free, high-contrast, high-speed imaging of microtubules using in vitro surfaces assays.

Tags

Keywords: Interference Reflection MicroscopyLabel-freeHigh-speed ImagingMicrotubulesHigh-contrastDICDark FieldFluorescence MicroscopyProtein ComplexesNanoparticles50/50 MirrorFilter CubeHigh Numerical ApertureParaffin FilmAnti-rhodamine AntibodyPoloxamer 407BRB80

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