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Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone

Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone

DOI:
10.3791/59537-v

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08:10 min

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June 24, 2019

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, , , ,
1Department of Microbiology and Immunology,University of Rochester Medical Center, 2Department of Molecular and Cell Biology,Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

Chapters

  • 00:04Title
  • 01:09Construction of a ZIKV Infectious cDNA Clone in a BAC
  • 01:41Preparation of High-purity pBAC-ZIKV for the Rescue of Infectious rZIKV
  • 02:36Rescue of Infectious rZIKV from the BAC cDNA Clone by Transfection of Vero Cells
  • 04:40Amplification and Generation of Viral Stocks
  • 05:58Results: Analysis of the Rescued Recombinant Zika Virus
  • 07:34Conclusion

Summary

Automatic Translation

Automatic Translation

The recent epidemic of Zika virus highlights the importance of establishing reverse genetic approaches to develop vaccines and/or therapeutic strategies. Here, we describe the protocol to rescue an infectious recombinant Zika virus from a full-length cDNA clone assembled in a bacterial artificial chromosome under the control of the human cytomegalovirus immediate-early promoter.

Tags

Keywords: Zika VirusReverse GeneticsBacterial Artificial ChromosomeCDNA CloneInfectious CloneRNA VirusFlavivirusViral BiologyViral PathogenesisVaccine DevelopmentTransfectionVero Cells

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