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Journal / Immunology and Infection / Redning af rekombinant Zikavirus fra en bakteriel …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Redning af rekombinant Zikavirus fra en bakteriel kunstig kromosom cDNA-klon
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Redning af rekombinant Zikavirus fra en bakteriel kunstig kromosom cDNA-klon

Article DOI: 10.3791/59537-v • 08:10 min • June 24th, 2019
June 24th, 2019 •
1, 1, 1, 2, 1
1Department of Microbiology and Immunology, University of Rochester Medical Center, 2Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

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Den nylige epidemi af Zikavirus fremhæver vigtigheden af at etablere omvendte genetiske tilgange til at udvikle vacciner og/eller terapeutiske strategier. Her beskriver vi protokollen til at redde en infektiøs rekombinant Zikavirus fra en fuld længde cDNA-klon samlet i et bakterielt kunstigt kromosom under kontrol af det humane Cytomegalovirus umiddelbare tidlige promotor.

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Immunologi og infektion Arbovirus flavivirus Zikavirus reverse genetik cDNA-infektiøse kloner i fuld længde bakterielle kunstige kromosom cDNA-transfection virus redning
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