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Genotyping of Sea Anemone during Early Development
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JoVE Journal Developmental Biology
Genotyping of Sea Anemone during Early Development

Genotyping of Sea Anemone during Early Development

DOI:
10.3791/59541-v

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07:04 min

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May 13, 2019

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,
1Department of Biological Sciences,University of Arkansas

Chapters

  • 00:04Title
  • 00:36Induction of Spawning, In Vitro Fertilization, and De-jellying
  • 01:48Surgical Removal of an Aboral Tissue from a Gastrula Embryo
  • 03:38Genomic DNA Extraction and Genotyping PCR
  • 04:54Results: Genotyping Results of Sea Anemone Embryos
  • 06:07Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to genotype the sea anemone Nematostella vectensis during gastrulation without sacrificing the embryo.

Tags

Keywords: GenotypingSea AnemoneEarly DevelopmentMutantEmbryogenesisPhenotypeNematostella VectensisSpawningFertilizationDejellyDNA ExtractionAgarose GelMicrosurgery

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