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JoVE Journal Cancer Research
Droplet Digital TRAP (ddTRAP)
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Droplet Digital TRAP (ddTRAP): anpassning av telomere upprepa amplifiering protokoll till droplet Digital polymerase kedje reaktion

Article DOI: 10.3791/59550-v • 06:38 min • May 3rd, 2019
May 3rd, 2019 •
1, 1, 1
1School of Kinesiology, University of Michigan

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Vi konverterade framgångs rikt standarden telomer upprepa amplifiering protokoll (trap) assay som skall användas i droplet Digital polymeras kedje reaktioner. Denna nya analys, som kallas ddtrap, är känsligare och kvantitativa, vilket möjliggör bättre detektion och statistisk analys av telomeras aktivitet inom olika mänskliga celler.

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Cancer forskning Telomerase TRAP-analys droplet Digital PCR cancer enzymatisk aktivitets detektering åldrande kvantitativ biologi
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