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Journal / Biochemistry / Enrichment of Native Lipoprotein Particles with…
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Biochemistry

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JoVE Journal Biochemistry
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
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Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Article DOI: 10.3791/59573-v • 11:13 min • May 9th, 2019
May 9th, 2019 •
1, 2, 1, 1, 2
1Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry, Medical University Vienna, 2School of Medical Engineering and Applied Social Sciences, University of Applied Sciences Upper Austria

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Summary
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Here, a quantitative real-time polymerase chain reaction-based protocol is presented for the determination of the native micro-RNA content (absolute/relative) of lipoprotein particles. In addition, a method for increasing the micro-RNA level, as well as a method for determining the cellular uptake rate of lipoprotein particles, is demonstrated.

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Enrichment Native Lipoprotein Particles MicroRNA Absolute MicroRNA Content Relative MicroRNA Content Cellular Transfer Rate Track Carrier Labeling Enrichment Methods Delipidation HDL Solution Centrifugation Tube Ethanol Diethyl Ether Mixture Nitrogen Gas Flow Resuspension Buffer A Protein Concentration
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