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JoVE Journal
Genetics

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Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
 

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

Article doi: 10.3791/59578
May 14th, 2019

Summary May 14th, 2019

A protocol to create gene modified human pseudoislets from dispersed human islet cells that are transduced by lentivirus carrying short hairpin RNA (shRNA) is presented. This protocol utilizes readily available enzyme and culture vessels, can be performed easily, and produces genetically modified human pseudoislets suitable for functional and morphological studies.

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