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Evaluation of the Storage Stability of Extracellular Vesicles
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Bioengineering
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JoVE Journal Bioengineering
Evaluation of the Storage Stability of Extracellular Vesicles

Evaluation of the Storage Stability of Extracellular Vesicles

DOI:
10.3791/59584-v

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11:31 min

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May 22, 2019

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, ,
1Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI),Biogenic Nanotherapeutics group (BION), 2Department of Pharmacy,Saarland University

Chapters

  • 00:04Title
  • 00:55Cell-Conditioned Medium (CCM) Preparation and Ultracentrifugation and Glucuronidase Encapsulation
  • 03:22Size-Exclusion Chromatography (SEC) Purification
  • 04:18Glucuronidase Assay
  • 05:13Extracellular Vesicle (EV) Lyophilization and Rehydration
  • 06:19EV Analysis After Storage
  • 09:15Results: Representative EV Storage Stability Evaluation
  • 10:47Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a readily applicable protocol to assess the storage stability of extracellular vesicles, a group of naturally occurring nanoparticles produced by cells. The vesicles are loaded with glucuronidase as a model enzyme and stored under different conditions. After storage, their physicochemical parameters and the activity of the encapsulated enzyme are evaluated.

Tags

Keywords: Extracellular VesiclesStorage StabilityGlucuronidaseAsymmetric Flow Field Flow Fractionation (AF4)Cell CultureUltracentrifugationBeta-glucuronidaseSaponin

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