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JoVE Journal
Genetics

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Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
 

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

Article doi: 10.3791/59630
May 13th, 2019

Summary May 13th, 2019

This molecular-based approach for determining bacterial fitness facilitates precise and accurate detection of microorganisms using unique genomic DNA barcodes that are quantified via digital PCR. The protocol describes calculating the competitive index for Salmonella strains; however, the technology is readily adaptable to protocols requiring absolute quantification of any genetically-malleable organism.

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