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JoVE Journal
Immunology and Infection

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In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
 

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

Article doi: 10.3791/59641
May 11th, 2020

Summary May 11th, 2020

Here we describe an indirect ELISA sandwich immunocapture to determine the immunogenic glycoprotein contents in rabies vaccines. This test uses a neutralizing Monoclonal Antibody (mAb-D1) recognizing glycoprotein trimers. It is an alternative to the in vivo NIH test to follow the consistency of vaccine potency during production.

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