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Journal / Neuroscience / 免疫組織化学用マウスレチンクライオ切片の調製…
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JoVE Journal Neuroscience
免疫組織化学用マウスレチンクライオ切片の調製
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免疫組織化学用マウスレチンクライオ切片の調製

Article DOI: 10.3791/59683-v • 05:25 min • July 1st, 2019
July 1st, 2019 •
1, 2, 2, 1
1Department of Biomedical Sciences, University of Pennsylvania School of Veterinary Medicine, 2Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine, University of Pennsylvania School of Veterinary Medicine

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本報告では、免疫組織化学(IHC)のための凍結マウス網膜切片を準備するための包括的な方法について説明する。 記載された方法は、眼後カップの解剖、パラホルムアルデヒド固定、最適切断温度(OCT)培媒体および組織配向への埋め込み、断断および免疫染色を含む。

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神経科学,第149号,網膜,クライオセクション,最適切断温度媒体,OCT,免疫組織化学,マウス眼のエグリュール化
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