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Assessment of Vascular Tone Responsiveness using Isolated Mesenteric Arteries with a Focus on Modulation by Perivascular Adipose Tissues
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Assessment of Vascular Tone Responsiveness using Isolated Mesenteric Arteries with a Focus on Modulation by Perivascular Adipose Tissues

Assessment of Vascular Tone Responsiveness using Isolated Mesenteric Arteries with a Focus on Modulation by Perivascular Adipose Tissues

DOI:
10.3791/59688-v

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08:41 min

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June 03, 2019

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, , , , , , ,
1The State Key Laboratory of Pharmaceutical Biotechnology and the Department of Pharmacology and Pharmacy,University of Hong Kong

Chapters

  • 00:04Title
  • 01:17Normalization to Determine the Optimal Initial Tension
  • 03:43Phenylephrine-induced Contractions
  • 05:52Results: Optimal Initial Tensions and Output Recordings of the Vasoconstrictor Responses to Phenylephrine in Mesenteric Arteries With or Without Surrounding PVAT
  • 07:34Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol describes the use of wire myography to evaluate the transmural isometric tension of mesenteric arteries isolated from mice, with special consideration of the modulation by factors released from endothelial cells and perivascular adipose tissues.

Tags

Keywords: Vascular ToneMesenteric ArteriesIsometric ConditionVascular ResponsivenessHypertensionCardiovascular ComplicationsMyographNormalizationPassive StretchWall TensionActive ForcePotassium Chloride

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