Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / A Uniform Shear Assay for Human Platelet and Cell …
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
Preparation and In Vitro Differentiation of Mesenchymal Stem Cell-Encapsulated Hydrogels for Bone Formation
Journal (Biology)
Preparation and In Vitro Differentiation of Mesenchymal Stem Cell-Encapsulated Hydrogels for Bone Formation
Preparing T Cell Growth Factor from Rat Splenocytes
Journal (Biology)
Preparing T Cell Growth Factor from Rat Splenocytes
 

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

Article DOI: 10.3791/59704-v • 04:32 min • June 5th, 2019
June 5th, 2019 •
1,2, 1,2, 1,2
1Aflac Cancer and Blood Disorders Center, Children's Healthcare of Atlanta, 2Department of Pediatrics, Emory University School of Medicine

Chapters

Summary
Automatic Translation

We describe an in-solution method to apply uniform shear to platelet surface receptors using cone-plate viscometry. This method may also be used more broadly to apply shear to other cell types and cell-fragments and need not target a specific ligand-receptor pair.

Tags

Uniform Shear Assay Human Platelets Cell Surface Receptors Cone-plate Viscometry Shear Duration Shear Magnitude Immobilized Cells Downstream Signal Detection Methods Peripheral Blood Platelet-rich Plasma Trisodium Citrate Pipette Tip Platelet Count Pooled Human Platelet-poor Plasma Ligand Treatment Antibody Ligand Of Interest Incubation Cone-plate Viscometer Plate Temperature
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter