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JoVE Journal Cancer Research
Vurdering cellelevedygtighed og død i 3D Spheroid kulturer af kræftceller
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Vurdering cellelevedygtighed og død i 3D Spheroid kulturer af kræftceller

Article DOI: 10.3791/59714-v • 10:33 min • June 16th, 2019
June 16th, 2019 •
1, 1, 1
1Section for Cell Biology and Physiology, Department of Biology, Faculty of Science, University of Copenhagen

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Her præsenterer vi flere enkle metoder til at evaluere levedygtighed og død i 3D cancer celle sfæroider, som efterligner de fysisk-kemiske gradienter af in vivo tumorer meget bedre end 2D-kulturen. Den sfæide model giver derfor mulighed for evaluering af kræft stof effektivitet med forbedret oversættelse til in vivo betingelser.

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Kræftforskning spheroider 3D cellekultur hængende drop cellelevedygtighed brystkræft kræft i bugspytkirtlen anti-cancer terapi narkotikabehandling kemoterapi rekonstitueret kælder membran propidium iodide immunhistokemi
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