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Journal / Immunology and Infection / Eksperimentel analyse af apoptotisk Thymocyt…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Eksperimentel analyse af apoptotisk Thymocyt engulfment af makrofager
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Eksperimentel analyse af apoptotisk Thymocyt engulfment af makrofager

Article DOI: 10.3791/59731-v • 06:47 min • May 24th, 2019
May 24th, 2019 •
1, 1
1Division of Immunology, Allergy, and Rheumatology, Department of Internal Medicine, College of Medicine, University of Cincinnati

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Her præsenterer vi en protokol til at forberede apoptotiske thymocytter og peritonealdialyse makrofager og analysere effektiviteten af efferocytose og den specifikke inhibitor-medieret blokering af apoptotiske thymocytter engulfment. Denne protokol har en bred anvendelse i celle-medieret clearance af andre partikler, herunder kunstige perler og bakterier.

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Immunologi og infektion udstedelse 147 makrofag apoptotiske celler efferocytose immuno Fluorescens mikroskopi flow cytometri TAM receptor tyrosinkinaser
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