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DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
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Biochemistry
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JoVE Journal Biochemistry
DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

DOI:
10.3791/59737-v

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08:04 min

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October 08, 2019

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, , , , ,
1Department of Chemistry,Ben-Gurion University of the Negev, 2Center for Genomic and Computational Biology, Department of Biostatistics and Bioinformatics,Duke University

Chapters

  • 00:04Title
  • 01:29Primase DNA Binding Experiment: Blocking Procedure
  • 02:44Primase DNA Binding Experiment: Protein Binding
  • 03:22Primase DNA Binding Experiment: Fluorescent Antibody Attachment
  • 05:15Results: Analysis of High-Throughput Primase Profiling
  • 06:42Conclusion

Summary

Automatic Translation

Automatic Translation

Protein binding microarray (PBM) experiments combined with biochemical assays link the binding and catalytic properties of DNA primase, an enzyme that synthesizes RNA primers on template DNA. This method, designated as high-throughput primase profiling (HTPP), can be used to reveal DNA-binding patterns of a variety of enzymes.

Tags

Keywords: DNA PrimaseProtein-DNA BindingMicroarrayHigh-throughputSequence RecognitionRNA Primer FormationTranscription FactorsDNA BindingEnzymatic ActivitySequence Determinants

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