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Biochemistry

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Use of Alu Element Containing Minigenes to Analyze Circular RNAs
 

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Article doi: 10.3791/59760
March 10th, 2020

Summary March 10th, 2020

We clone and analyze reporter genes generating circular RNAs. These reporter genes are larger than constructs to analyze linear splicing and contain Alu elements. To investigate the circular RNAs, the constructs are transfected into cells and resulting RNA is analyzed using RT-PCR after removal of linear RNA.

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