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Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells

Real-Time, Semi-Automated Fluorescent Measurement of the Airway Surface Liquid pH of Primary Human Airway Epithelial Cells

DOI:
10.3791/59815-v

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10:18 min

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June 13, 2019

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, , , , , ,
1Epithelial Research Group, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences,Newcastle University, 2Respiratory Group, Institute of Cellular Medicine, Faculty of Medical Sciences,Newcastle University, 3Paediatric Respiratory Medicine, Great North Children’s Hospital,Newcastle upon Tyne Hospitals NHS Foundation Trust, 4Boehringer Ingelheim Pharma GmbH & Co

Chapters

  • 00:04Title
  • 01:09Primary Human Airway Epithelial Cell (hAEC) Preparation
  • 01:53Background Measurement
  • 04:43Kinetics Measurement
  • 05:45In Situ pH Calibration
  • 06:28Data Analysis
  • 07:58Results: Representative pH Calibration in Primary hAECs In Situ
  • 09:34Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to make dynamic measurements of the airway surface liquid pH under thin film conditions using a plate-reader.

Tags

Keywords: Real-timeSemi-automatedFluorescent MeasurementAirway Surface Liquid PHPrimary Human Airway Epithelial CellsIon ChannelsTransportersExtracellular PH RegulationCystic FibrosisAsthmaCOPDAir-liquid InterfaceBicarbonate BufferPlate ReaderTemperature ControlCarbon Dioxide ControlHumidity CassetteKinetic Loop

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